Association of haemato-biochemical indices and blood composite ratios with microfilaridermia in Onchocerciasis patients.

BACKGROUND: Onchocerciasis causes chronic systemic inflammation. Several studies have used markers such as haemato-biochemical indices to predict the occurrence of systemic inflammation. This study assessed the variability and predictability of haemato-biochemical indices and blood composite ratios (BCRs) in microfilariae positive (MF+) and microfilariae negative (MF-) subgroups of onchocercomata participants. METHODS: One hundred and five (105) MF + and 34 MF- participants were retrospectively recruited into the study. Screening for the presence of O. volvulus microfilariae was done from skin snips taken from the left and right iliac crests of participants using established and approved protocols. Haematological and biochemical indices were measured using standard laboratory automated analyzers. Blood composite ratios (BCRs) were calculated as ratios of the absolute parameters involved. RESULTS: A significantly increased total WBC, absolute eosinophil, eosinophil percent and absolute basophil were observed in the MF + participants compared to MF- participants. Reduced gamma-glutamyl transferase (GGT) with increased estimated glomerular filtration rate (eGFR) was significantly associated with MF + participants compared to MF- participants. BCRs were significantly higher for eosinophil-to-neutrophil ratio (ENR), eosinophil-to-monocyte ratio (EMR), eosinophil-to-basophil ratio (EBR) and eosinophil-to-lymphocyte ratio (ELR) in MF + participants compared to MF- participants. After multivariate adjustment, onchocercomata participants with increased eosinophil counts (aOR = 13.86, 95% CI [2.07-92.90], p = 0.007), ENR x10 (aOR = 1.42, 95% CI [1.05-1.93], p = 0.025), EMR (aOR = 2.64, 95% CI [1.25-5.60], p = 0.011), EBR (aOR = 1.07, 95% CI [1.01-1.10], p = 0.020) and ELR x10 (aOR = 1.69, 95% CI [1.14-2.51], p = 0.009) were more likely to have microfilaridermia. CONCLUSIONS: Elevated eosinophil counts with higher ENR, EMR, EBR and ELR levels are significantly associated with microfilaridermia in onchocercomata participants. Combining BCRs with eosinophil count significantly led to an improvement in the conventional model for predicting microfilaridermia.

Soil transmitted helminth infections in Ghana: a ten year review.

For more than a decade, intervention programs have been instituted in Ghana to combat soil transmitted helminth (STHs) infections. Knowledge of the trend of the infection in the country is needed for evaluation and modification of existing control programs to achieve national targets. The objective of this review is to examine the pattern of soil transmitted infections in Ghana between 2009-2018. We searched and reviewed published literature on soil transmitted helminths in Ghana in PubMed, Medline, Google Scholar and Institutional Repositories of Kwame Nkrumah University of Science and Technology, University of Ghana, University of Cape Coast, and University for Development Studies-Tamale. We observed paucity of research work on STHs in Ghana over the period of this review. Twenty-nine studies consisting of 24 published works in peer reviewed journals and five graduate theses were included in the study. Hookworm was the most prevalent of STHs recorded followed by roundworm, threadworm, and whipworm. Pinworm was very rarely reported. These infections were reported from different regions and ecological zones of the country and among children, pregnant women, farmers, food vendors, children in orphanage home and psychiatric institution. Although there is some downward trend over the period, soil transmitted helminths are still prevalent in Ghana. This is an indication of some hope of eventual control and elimination of these diseases in the country if control measures are optimised. Further research particularly population studies into soil transmitted helminths in Ghana is needed.